Description
CST's PathScan® Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235 detects PDGF receptor α/β when tyrosine phosphorylated. As shown in Figure 1, a significant induction of PDGF Receptor α/β tyrosine phosphorylation can be detected in MG63 cells following treatment with PDGF using the Phospho-PDGF Receptor α/β (panTyr) Sandwich ELISA Kit #7235. The level of total PDGF receptor α (phospho and nonphospho) remains unchanged as shown by Western analysis and by PathScan® Total PDGF Receptor α Sandwich ELISA Kit #7318. Western blot analysis of protein captured in the PDGF receptor α/β antibody coated microwell indicates that PDGF receptor α/β (phospho and nonphospho) has been captured (data not shown). The Western blot also shows a major band corresponding to the phospho-PDGF receptor α/β protein when biotinylated anti-tyrosine antibody is used as probe (see Figure 3). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species